2.1. Materials

Rabbit anti-PARN antibody (ab125185 and ab188333) and mouse anti-PDI antibody were purchased from Abcam (Cambridge, MA, USA). Rabbit anti-PARN antibody (A6941) was obtained from Abclonal (Cambridge, MA, USA). Rabbit anti-MAPKAPK-2 antibody (#3042) was from Cell Signaling Technology (Beverly, MA, USA). Mouse antibodies against calnexin, CNOT6, and CNOT7 were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Mouse anti-GAPDH antibody was from Bioworld (Louis Park, MN, USA). Rabbit antibodies against LaminA/C, CNOT7, CNOT8, PAN2, MDM2, and RPS3 were from Proteintech (Chicago, IL, USA). The anti-Flag mouse antibody and anti-PNLDC1 rabbit antibody were from Sigma-Aldrich (St Louis, MO, USA). Rabbit antibodies against CDKL1, CCNT2, CLOCK, and MATR3 were purchased from BBI Life Sciences Corp. (Shanghai, China). Horseradish peroxidase (HRP)-conjugated secondary antibodies for Western blot were obtained from Yeasen (Shanghai, China). HRP-labeled sreptavidin for biotin-labeled RNA detection was from Beyotime (Beijing, China). Dylight 488/549/594/649 goat-anti-rabbit/mouse secondary antibodies for immunofluorescence observations were from Bioworld. The transfection reagent Lipofectamine™ and the RNAiMAX Reagent were from Invitrogen (Carlsbad, CA, USA) and Vigofect was from Vigorous (Beijing, China). Doxorubicin hydrochloride (DOX) was from Solarbio (Beijing, China). Cycloheximide (CHX) was from AMRESCO (Solon, OH, USA) and was dissolved in DMSO (Sigma-Aldrich, St Louis, MO, USA). Digitonin, hydroxyurea (HU), urea, and protease inhibitor cocktail used for mammalian cellular extraction were from Sigma. CMPD1 was purchased from Santa Cruz. Recombinant RNasin^® ribonuclease inhibitor and sequencing grade modified trypsin were purchased from Promega (Madison, WI, USA). RNase A/T1 Mix and yeast total RNA were from Thermo Scientific (Waltham, MA, USA). NP-40, DNase/RNase-free sucrose and sodium deoxycholate (DOC) were from AMRESCO. Oligo (dT)₂₅ cellulose beads for mRNA isolation and the low range ssRNA ladder were purchased from New England Biolabs (Ipswich, MA, USA). Sodium dodecyl sulfate (SDS) and paraformaldehyde were from Merck (Darmstadt, Germany). 1,2-dipalmitoyl-sn-glycero-3-phospho-L-serine (sodium salt) (DPPS) was from Avanti Polar Lipids (Alabaster, AL, USA). The kit for cytosolic and membrane proteins extraction was from KeyGen biotech (Nanjing, China). The kits for RNA 3′end biotin labeling and chemiluminescent nucleic acid detection were from Thermo Scientific. Viewsolid synthesized RNA oligos for PARN knockdown [7]. Takara synthesized Polyadenylate A₂₀ and A₂₀₀ was from Sigma. All other chemicals were of analytical grade.