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Vero cells growing in 12 well culture plates were prechilled at 4 °C for 5 min and were infected with 200 PFU/well of HSV-1 in the presence of the drug at 4 °C for 30 min. The medium was aspirated, and cells washed with PBS two times to remove unadsorbed virus. Culture medium containing 1% methylcellulose without drug were overlaid on cell monolayer, and cells were cultured for 2 days. Cells were stained with 1% crystal violet, and the total number of plaques was counted.
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