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Bacterial strains and growth media.

PK Priyanka Kachroo
JE Jesus M. Eraso
RO Randall J. Olsen
LZ Luchang Zhu
SK Samantha L. Kubiak
LP Layne Pruitt
PY Prasanti Yerramilli
CC Concepcion C. Cantu
MS Matthew Ojeda Saavedra
JP Johan Pensar
JC Jukka Corander
LJ Leslie Jenkins
LK Lillian Kao
AG Alejandro Granillo
AP Adeline R. Porter
FD Frank R. DeLeo
JM James M. Musser
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For in vitro growth, GAS strains were grown in Todd-Hewitt broth (Becton, Dickinson [BD]) supplemented with 0.2% yeast extract (THY medium). This medium was used (i) because it is a standard medium for GAS growth used by many investigators and (ii) because there is no medium that adequately mimics in vivo conditions. THY medium was supplemented with spectinomycin (150 μg ml−1) as needed. Escherichia coli strains were grown in Luria-Bertani (LB) medium at 37˚C. LB medium was supplemented with spectinomycin (50 μg ml−1), as needed. Trypticase soy agar supplemented with 5% sheep red blood cells (Becton, Dickinson and Co.) was also used for growth of GAS strains. Spectinomycin dihydrochloride pentahydrate was purchased from Sigma. Gap electroporation cuvettes (2-mm gap size) were purchased from BTX Harvard apparatus. Growth experiments were performed as described previously (93). Locus tag equivalence between strains MGAS5005 and MGAS2221 is shown in Table S1J in the supplemental material.

Copyright and License information:  ©2020 Kachroo et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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