Tumor regeneration and therapeutic experiments

Tumor transplantations were performed as previously described [9, 25]. For subcutaneous tumor experiments, we usually do two injections per mouse, 5 - 10 animals per group. For therapeutic experiments, DU145 cells were infected with negative control (lenti-Ctrl) and miR-199a-3p lentivirus (lenti-199A) and subcutaneously implanted into NOD/SCID female mice. When tumors became palpable, two groups of mice were randomly divided into two subgroups, each one of which was administrated with the doxycycline in the food (2 μg). Tumor volume was then measured every 2 - 3 days for approximately two months.