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Total RNA of transfected cells was prepared, and 2 μg of total RNA was reverse transcribed into cDNA using the PrimeScript™ 1st Strand cDNA Synthesis Kit (Takara) according to the manufacturer’s protocol. Quantitative real-time PCR was performed in triplicate on an ABI 7500 Real-Time PCR system using SYBR Green Master Mix (Takara). The mRNA levels were normalized to the expression of the housekeeping gene β-actin.
Copyright and License information: The Author(s). ©2020
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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