The need for Na2HPO4, H2O2, and NH3 to create GO/Ag nanocomposite was assessed by lowering Na2HPO4 concentration (25 mM, 10 mM, 5 mM, 2.5 mM, 0.5 mM, and 0 mM) in the presence of 40 µg GO, 3.2 mM AgNO3, 100 mM H2O2, and 40 mM NH3 in total 1 ml solution. Particularly, the 0 mM Na2HPO4 (i.e., ddH2O) experiment was performed with 40 µg GO and 3.2 mM AgNO3 in the presence and absence of 100 mM H2O2 and 40 mM NH3 at pH 4.0 and 37 °C. Without Na2HPO4, H2O2, and NH3, GO will depend on its natural abilities25 to convert aqueous AgNO3 to Ag nanoparticles.
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