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Behavioural equipment

JL Jonathan L. C. Lee
FA Felippe E. Amorim
LC Lindsey F. Cassini
OA Olavo B. Amaral
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The conditioning chambers (MedAssociates, VT) consisted of two identical illuminated boxes (25 cm × 32 cm × 25.5 cm), placed within sound-attenuating chambers. The box walls were constructed of steel, except by the ceiling and front wall, which were made of perspex. The grid floor consisted of 19 stainless steel rods (4.8 mm diameter; 1.6 mm centre-to-centre), connected to a shock generator and scrambler (MedAssociates, VT). Infrared video cameras were mounted on the ceiling of the chambers (Viewpoint Life Sciences, France) and used to record and quantify freezing behaviour automatically.

Copyright and License information:  ©2019 Lee et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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