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2.5. Circular Dichroism Analysis of Double-Stranded Oligonucleotides

BK Bolesław T. Karwowski
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CD spectra were recorded with a Jasco J-815 dichrograph (JASCO, Easton, MD, USA) at room temperature using 0.1 cm quartz cuvettes and an oligonucleotide concentration of 0.5 μM, as this concentration permitted parallel UV measurements. The CD spectra were collected within the range of 200 to 400 nm with an integration time of 1 s, increment 1 nm, band width 1 nm, scanning speed 50 nm/min. The profiles of the obtained spectra are given in Figure S1.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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