Chemicals and reagents

We obtained 5-fluorouracil (99%) from the Zhenzhou Huawen Chemical Co., Ltd. (Zhenzhou, China). Gallic acid (<98%), catechinic acid (98%) and ellagic acid (98%) were purchased from Chengdu PUSH Bio-technology Co., Ltd. (Chengdu, China). The CCK8 kit was from Dojindo (Kumamoto, Japan). The Hoechst 33258 staining solution, the mitochondrial membrane potential assay kit with JC-1, the Reaction Oxygen Species Assay Kit and N-acetyl-L-cysteine (NAC) were purchased from Beyotime (Jiangsu, China). The Annexin V-FITC Apoptosis Detection kit and the PI/RNase Staining Buffer were obtained from BD Biosciences (New Jersey, USA). The Caspase-3 and Caspase-9 Colorimetric Assay Kit, together with Z-DEVD-FMK, Z-LEHD-FMK and Cytochrome c Apoptosis Assay Kit were purchased from Biovision, Inc. (Milpitas, CA). The primary antibodies against Bcl-2 and the secondary antibody were obtained from EMD Millipore Corporation (Temecula, CA). Anti-Bax was from Abcam (Cambrige, MA, USA) and anti-β-actin and anti-HSP 90 were from Cell Signaling Technology (Beverly, MA, USA). Anti-cleaved caspse-3/9 and anti-cleaved PARP, and anti-LC3B and anti-p62 were a kind gift from Dr. Hongsheng Wang and Dr. Min Li (Sun Yat-sen University), respectively.