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Plat E cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (HyClone Laboratories, Logan, UT, USA) supplemented with 10% FBS, puromycin (1 μg/mL) and blasticidin (10 μg/mL) every three days. Cells were plated onto either 100 mm dishes (1.3 × 106 cell/dish) or 6-well plates (3 × 105 cells/well) one day prior to transfection.
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