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To degrade unprotected RNA and ascertain the isolation of RNA only confined within EVs, purified EV pellets were treated with RNase A (0.1/0.5 mg/mL; Thermo Fisher Scientific) for 20 min at 37 °C, followed by enzyme inactivation. Untreated purified EVs were tested in parallel as controls.
Copyright and License information: ©2019 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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