CD20 (MS4A1) guilt-by-association analysis

Probes representing MS4A1 were collapsed according to the mean. Next, we used mRNA signals to determine the association of each gene with the expression pattern of MS4A1. The association was determined by the Pearson correlation coefficient. Gene set enrichment analyses (GSEA) were performed on the 500 top-ranked MS4A1-associated probes (390 unique genes). The 390 MS4A1 co-expressed genes were uploaded to Enrichr [25], and several gene set databases were consulted (KEGG, Wiki pathways, Biocarta, NCI Nature, Panther and GO biological process). To annotate a single gene to only one biological pathway, we manually marked single genes to 9 different biological pathways (BCR signaling, cytoskeleton regulation, DNA repair and cell cycle, histone modification, immune regulation, metabolism, protein processing, RNA processing, signaling protein (not further specified)).