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Images of brain sections were acquired using a Nikon AR1 confocal microscope. Representative sections from three cases of each condition covering the centre of injection sites were imaged. Delivery area and cell counts were estimated using ImageJ software. Briefly, the area with fluorescence above the threshold (mean background) was calculated, and all cells within the delivery area were counted with ImageJ. Data were analysed using one‐way ANOVA followed by Turkey's post hoc test. P < 0.05 was considered statistically significant.
Copyright and License information: ©2019 The Authors Published by John Wiley & Sons Ltd
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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