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HoThyRu/DOTAP and HoThyDansRu/DOTAP liposome preparation

MP Marialuisa Piccolo
GM Gabriella Misso
MF Maria Grazia Ferraro
CR Claudia Riccardi
AC Antonella Capuozzo
MZ Mayra Rachele Zarone
FM Francesco Maione
MT Marco Trifuoggi
PS Paola Stiuso
GD Gerardino D’Errico
MC Michele Caraglia
LP Luigi Paduano
DM Daniela Montesarchio
CI Carlo Irace
RS Rita Santamaria
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The here investigated ruthenium(III) complex, named HoThyRu, was prepared by reacting in stoichiometric amounts the starting nucleolipid HoThy (Fig. S2) with the Ru complex [trans-RuCl4(DMSO)2]−Na+ following a previously described procedure27,30,64. A similar procedure was followed to prepare the fluorescently labelled complex HoThyDansRu starting from the nucleolipid HoThyDans (Fig. S3), as previously described30. The desired nucleolipidic Ru(III) complexes were obtained in a pure form, as confirmed by TLC and ESI-MS analysis, and almost quantitative yields. The lipid formulations of HoThyRu and HoThyDansRu in DOTAP were prepared as previously reported and characterized by DLS analysis28,30.

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