Investigation of signal suppression

MK Maria Kaltenbrunner
WM Walter Mayer
KK Kirsten Kerkhoff
RE Rita Epp
HR Hermann Rüggeberg
RH Rupert Hochegger
MC Margit Cichna-Markl
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Signal suppression, observed for assayChr1D and assayChr1W, was investigated by analysing a DNA mixture (20 ng/µL) containing 25% (w/w) target subspecies DNA in non-target subspecies DNA as background and serial dilutions (1:4 to 1:1,024) thereof in water. The positive control contained 100% (w/w) target subspecies DNA (5 ng/µL) and had the same target subspecies DNA concentration as the 1:4 diluted standard. The extent of signal suppression was evaluated by comparing the maximum ΔRn values.

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