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Inducible knockdown of P. berghei pTKL
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The degradation of PbpTKL-AID-HA in the presence of 3-indoleacetic acid (IAA, Sigma-Aldrich) was first analyzed by western blot. Nycodenz-enriched infected erythrocytes, with or without exposure to 0.5 µM IAA for 30 min at 37 °C, were lysed (total lysis protocol) and PbpTKL-AID-HA was immunoprecipitated. After confirming protein degradation in the presence of IAA, the intra-erythrocytic cycle was studied in vitro as described above, except that rings were cultured with or without 0.5 µM IAA57.
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