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Gene expression levels within biofilms via atpB::gfp reporter
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For measurement of atpB promoter activity of bacterial cells within the microcolony, the biofilms were sequentially scanned using the 488 nm Argon laser to minimize the crosstalk between GFP and Lysosensor, and the fluorescence emitted was collected with the internal spectral detectors (515–545 nm). For visualization of gene expression level, ImageJ’s Green LUT was applied (Fig. 7).
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