Quantitative PCR.

cDNA was synthesized from total RNA using an oligo(dT) primer (TransGen) and subjected to quantitative PCR using SYBR green PCR master mix (Toyobo). The relative amounts of the indicated RNAs were calculated using β-actin as an internal control. The primer pairs used were as follows: primer pair lnc-ISG20-F (5′-GCATCCCGACATTGGTTTA-3′) and lnc-ISG20-R (5′-AGCTGCAGGATCTACTTACAGAC-3′) and primer pair ISG20-F (5′-TGGACTGCGAGATGGTGG-3′) and ISG20-R (5′-GGGTTCTGTAATCGGTGAT-3′).