RNAseq of near-isogenic SIL fruit

Noam Chechanovsky; Ran Hovav; Rina Frenkel; Adi Faigenboim; Yelena Eselson; Marina Petreikov; Michal Moy; Shmuel Shen; Arthur A. Schaffer

Improve Research Reproducibility A Bio-protocol resource

Home
Protocols

Concise Method

RNAseq of near-isogenic SIL fruit

NC Noam Chechanovsky

RH Ran Hovav

RF Rina Frenkel

AF Adi Faigenboim

YE Yelena Eselson

MP Marina Petreikov

MM Michal Moy

SS Shmuel Shen

AS Arthur A. Schaffer

This method is extracted from research article: Hortic Res, Nov 2019

Low temperature upregulates cwp expression and modifies alternative splicing patterns, increasing the severity of cwp-induced tomato fruit cuticular microfissures

DOI: 10.1038/s41438-019-0204-9

Ask a question

Favorite

For the study of global gene expression during fruit development, the SIL line segregants were grown, and fruit pericarp and peel were sampled from immature green (IG), mature green (MG), and breaker (Br) stages. For the study of the effect of temperature on detached fruitlets, IG fruitlets of MT BC₃F₇ lines were sampled and kept for 10 h, either at 20 or at 4 °C. Two replications, each of a minimum of three fruits, were prepared for each MT genotype. Tissues containing the skin and outer pericarp were sampled and immediately frozen in liquid nitrogen. Samples were prepared for RNAseq and run on Illumina HiSeq2000, as described in Shammai et al.⁴⁴.

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

Post a Question

0 Q&A

Share your protocol with your peers.

Submit a Preprint Protocol