Statistical analysis

All results are expressed as means±SEM, if not stated otherwise. Data were statistically analyzed with GraphPad Prism 6 for Windows by using two-way ANOVA (repeated measures) with Sidak's multiple comparison test. Spearman's rank correlation coefficient was calculated to estimate the associations between circulating levels of miRNAs, cTnI and changes in LVEF (ΔLVEF/LVEF_baseline). Receiver operator characteristic (ROC) analysis was generated to determine the ability of miRNA and cTnI levels to discriminate between cardiotoxicity and no-cardiotoxicity patients. The difference between the ROC curves was compared using DeLong test in MedCalc v.16.8.4. All tests were performed 2-tailed, and a significance level of p<0.05 was considered to indicate statistical significance. To verify whether studied miRNAs were differentially expressed in patients with cardiotoxicity, we grouped those patients who met the cardiotoxicity criteria (described above) as “cardiotoxicity group” and compared them with the others (“non-cardiotoxicity group”).