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Plasmid constructs, lentivirus production, and cell transfection
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Full-length human CD133 was subcloned into pWPXL (Addgene, Cambridge, Massachusetts, USA) plasmid as previously described22. A generic negative control (NC) sequence was synthesized by GenePharma (Shanghai, China). CXCL3 and NC shRNA were subcloned into pLVTHM (Addgene, USA) as described on the Addgene website. Virus packaging and cell transfection were performed as previously described22. Primers for cloning and the CXCL3 promoter sequence are provided in Supplementary Tables S7–S9.
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