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Protein samples were resolved by 10% SDS-PAGE, transferred to a polyvinylidenefluoride membrane and immunoblotted. After two hours of blocking in 5% dry-milk in TBS-T, Primary antibody was applied overnight at 4 °C followed by incubation with a suitable HRP-coupled secondary antibody (1:10000) (Santa Cruz, Heidelberg, Germany). Quantitative densitometric analysis was carried out with ImageJ/Fiji version 1.46.
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