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Mutation of STAT3 binding site on VEGF-A promoter
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The mutagenic constructs were made using the QuickChange II XL Site-Directed Mutagenesis Kit (Stratagene). For this, the mutagenic primers with mutations in STAT3 binding site were synthesized by the MDBio. The sequences were as follow: F: 5′-CCCTGGACACTTCCGTTAGGACCCCAGT-3′, and R: 5′-ACTGGGGTCCTAACGGAAGTGTCCAGGG-3′.
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