Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
The 96-well plate design included increasing concentrations of known concentrations of ETH or 20-E in EIA buffer + NGS or BSA, respectively, in triplicate. 3’,3’,5’,5’-tetramethylbenzidine (TMB) peroxidase substrate + peroxidase (KPL) was added to each well and allowed to react for ~ 12 min until the reaction was terminated with 1 M phosphoric acid. The absorbance at 450 nm was immediately read, and the absorbance of standards was plotted and interpolated using a four-parameter logistic equation, used to predict concentrations of unknowns.
Copyright and License information: Adams et al. ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
