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MRI evaluation of tumor growth and survival

SS Susobhan Sarkar
CP Candice C. Poon
RM Reza Mirzaei
KR Khalil S. Rawji
WH Walter Hader
PB Pinaki Bose
JK John Kelly
JD Jeffrey F. Dunn
VY V. Wee Yong
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Between 7 to 10 weeks post-implantation, an MRI of the brain was conducted at the University of Calgary Experimental Imaging Center as previously described27 using a 9.4 T Brunker horizontal-bore MR system. Images were analyzed using Marevici software.

We also performed survival analysis with Kaplan–Meier curves. Survival endpoints complied with protocols approved by the Animal Care Committee at the University of Calgary in accordance with research guidelines from the Canadian Council for Animal Care. They were defined as mice that were moribund (negligible limb movement and loss of 25% of body weight compared to the preceding few days) or mice that were found deceased in their cages. Upon sacrifice, brains were removed and immersed in 10% buffered formalin, paraffin-embedded and sectioned for histology.

Copyright and License information: The Author(s) ©2018
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