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The MeDIP experiment was performed as previously described17. Briefly, the genomic DNA was purified and sonicated to size of 200–500 bp. DNA fragments were denatured and incubated with antibody against 5mC (Eurogentec), 5hmC (Active Motif), or control IgG(Abcam, ab37415) at 4 °C overnight. The precipitated DNA fragments were analyzed by qPCR. Primers used for MeDIP-qPCR are listed in Supplementary Table 3. MeDIP seq data were described before17.
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