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Peptide preparation

LS Limin Song
LP Lei Pei
LH Lisha Hu
SP Shangwen Pan
WX Wei Xiong
ML Min Liu
YW Yan Wu
YS You Shang
SY Shanglong Yao
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Aβ25–35 and Aβ1–42 (ChinaPeptides Co., Shanghai, China) were prepared as previouly described22,54,55. The Aβ25–35 was dissolved in sterile double-distilled water (3 mM) and incubated at 37 °C for 4 days to form “aged” Aβ25–35. To form Aβ1–42 fibrils, Aβ1–42 was dissolved in double-distilled water (10 mM) and incubated at 37 °C for 24 h. To prepare Aβ1–42 oligomers, Aβ1–42 was dissolved in dimethyl sulfoxide (DMSO, St. Louis, MO, USA) (5 mM) and incubated at 4 °C for 24 h. Aβ aggregates were diluted to the final concentration with phosphate buffer saline (PBS) just before each experiment.

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