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2.12. Immunofluorescence (IF) staining
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We performed IF staining as previously described [23]. After incubated with anti-c-Fos antibody (Santa Cruz Biotechnology, CA, USA; 1:500) overnight, brain slides were treated with Alexa Fluor 555 and 488–conjugated anti-rabbit antibody (Invitrogen, MA, USA) at room temperature for 1 h. Images were taken by a Zeiss LSM 510 confocal microscope (Carl Zeiss Imaging, Oberkochen, Germany).
Copyright and License information: ©2018 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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