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Transient transfection

CL Cong Liu
LL Lian Liu
HZ Hai-Dan Zhu
JS Jia-Qi Sheng
XW Xiao-Li Wu
XH Xing-Xing He
DT De-An Tian
JL Jia-Zhi Liao
PL Pei-Yuan Li
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L02 cells were transfected with RFP-GFP-LC3 double fluorescence lentivirus (#GPL2001, Genechem, Shanghai, China) at 20–30% confluence according to the manufacturer’s protocol. We got the stable expression transfectant by adding puromycin. Then the L02 cells were treated with PA or celecoxib. To knockdown COX-2, siRNAs were transfected into cells using Lipofectamine2000 (#11668-019, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. The siRNA sequences (#1299001, Invitrogen, Carlsbad, CA, USA) used as follow: si-1 5′-GAAGCCUUCUCUAACCUCUCCUAUU-3′ and si-2 5′-AUCAGUAUUAGCCUGCUUGUCUGGA-3′.

Copyright and License information: The Author(s) ©2018
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