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Annexin V apoptosis assay

SR Suhail Ahmed Kabeer Rasheed
HL Hui Sun Leong
ML Manikandan Lakshmanan
AR Anandhkumar Raju
DD Dhivya Dadlani
FC Fui-Teen Chong
NS Nicholas B Shannon
RR Ravisankar Rajarethinam
TS Thakshayeni Skanthakumar
ET Ern Yu Tan
JH Jacqueline Siok Gek Hwang
KL Kok Hing Lim
DT Daniel Shao-Weng Tan
PC Paolo Ceppi
MW Mei Wang
VT Vinay Tergaonkar
PC Patrick J. Casey
NI N. Gopalakrishna Iyer
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Tissue culture conditions and drug treatments were performed as described [49]. 5-Fluorouracil, cisplatin, paclitaxel and doxorubicin were purchased from Sigma-Aldrich (St. Louis, MO, USA). 1 × 105 Cells were seeded in six well plates followed by treatment with 10 μM Cisplatin, 300 μM 5-Fluorouracil, 0.05 μM Paclitaxel and 0.345 μM doxorubicin for 72 h. For γ-Irradiation, the cells were treated once with 15 Gy of ɣ-radiation using Gamma cell 40 exactor (Best Theratronics, Ontario, Canada) and incubated for 72 h. Annexin V staining was performed as recommended by manufacturer (Life Technologies, Netherlands). The samples were read out using FACSCanto II (BD Biosciences, San Jose, CA, USA) and analyzed with FACSDiva software (BD Biosciences) within 4 h.

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