Animals

All animal experiments were approved by the Mayo Clinic Institutional Animal Care and Use Committee (IACUC) and were carried out in accordance with IACUC policy and the Guide for the Care and Use of Laboratory Animals.21 A total of seven domestic swine of Landrace origin (n = 5 female and n = 2 male) were administered pIL-10/AAV8 in two cohorts. The first cohort consisted of five animals (namely, Y872, Y876, Y877, Y965, and Y971). The second cohort consisted of two animals (namely, Y342 and Y343). At the time of vector administration, animals were 4 months old and weighed between 23.0 and 30.5 kg. Animals were closely observed for overall appearance, appetite, activity, changes in behavior, body temperature, weight, heart rate, and respiratory rate. The study duration was 56 days after vector administration, at which point the primary endpoint was met and animals were humanely euthanized. Animals that became moribund satisfied the humane endpoint according to institutional policy and were humanely euthanized prior to completing 56 days of study. Institutional and IACUC rules mandate euthanasia of animals that are destined to die from dehydration and subsequent systemic organ failure. Therefore, neurotoxicity is described as fatal in this article.