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Western Blotting

JL Jane Levesley
LS Lynette Steele
AB Anke Brüning-Richardson
AD Adam Davison
JZ Jia Zhou
CD Chunyong Ding
SL Sean Lawler
SS Susan C Short
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Cell lysates were prepared and separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and probed with antibodies against poly(ADP-ribose) polymerase (PARP), Lamin A/C, BCL-XL, BCL-2, BCL-w, BAX, BAK, Bid, Puma, Bad, p-Bad (Ser112), Bik, Bim, phospho–Aurora A (Thr288)/phospho–Aurora B (Thr232)/phospho–Aurora C (Thr198), and total Aurora A and B (Cell Signaling Technologies). Antibody binding was detected using the Odyssey imaging system (LI-COR Biosciences) or the Supersignal West Pico Mouse and Rabbit IgG detection kits (Life Technologies). Equal loading was confirmed using a β-actin antibody (Sigma-Aldrich).

Copyright and License information:  ©2017 The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

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