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T1L/T3D M2, T1L/T3D M2 Δ341, T1L/T3D M2 Δ342, T1L/T3D M2 V403A, T1L/T3D M2 Δ341 V403A, or T1L/T3D M2 Δ342 V403A virions or ISVPs (2 × 1012 particles/ml) were analyzed using a Zetasizer Nano S dynamic-light-scattering system (Malvern Instruments, Malvern, United Kingdom). All measurements were made at room temperature in a quartz Suprasil cuvette with a 3.00-mm path length (Hellma Analytics, Mullheim, Germany). For each sample, the hydrodynamic diameter was determined by averaging readings across 15 iterations.
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