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PCR array analysis

AT Anita Thyagarajan
JS Jeremiah Saylae
RS Ravi P. Sahu
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To determine the ASA target(s), B16-MSCV tumors from vehicle and ASA-treated WT mice (n=10 mice/group) were harvested for PCR array analysis. Briefly, total RNA was extracted and 1μg of total RNA from each tumor sample was pooled separately from each group. Complementary DNA (cDNA) was synthesized using cDNA synthesis kit and blotted separately into RT2 ProfilerTM PCR array plates (PAMM-176Zc-2 for mouse cancer stem cells) and qPCR and analysis were performed as per manual's protocol and software.

Copyright and License information:  ©2017 Thyagarajan et al.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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