Sample binding and processing

The microarrays were incubated overnight at 4 °C with individual antivenom samples mixed with binding buffer at either 1:50 or 1:100 dilution to a final volume of 100 μL. This was followed by three 10 minute washes with a TBST (Tris Buffered Saline and Tween 20) buffer and incubating with goat anti-horse IgG (H+ L) conjugated with Alexa Flour^® 647 (Jackson ImmunoResearch, 108-605-003) at room temperature for 3 hours. After a final wash, the arrays were dried and read using an MS200 microarray scanner, and signals were extracted using NimbleGen DEVA signal extraction software