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After 24 h, the cell suspension was carefully centrifuged and cell culture supernatants collected for subsequent IL-10 analysis. IL-10 expression was measured using a commercially available IL-10 enzyme-linked immunosorbent assay (ELISA; R&D systems), according to the manufacturer’s instructions.
Copyright and License information: The Author(s) ©2017
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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