pGL2-Basic-RPE65(−450/+39) was a kind gift from Debra Thompson (University of Michigan). pGL3-Basic-LRAT(−268/+257) was a kind gift from Catharine Ross (University of Pennsylvania). pGL2-Basic-RPE65(−450/+39) (33) was digested with BglII and StuI and religated in order to remove the AP-1 site (190 bp segment from the start codon).
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