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Enzyme-linked immunosorbent assay

CH CK Matthew Heng
ID Ilona Darlyuk-Saadon
WL Wupeng Liao
MM Manju P Mohanam
PG Phyllis XL Gan
NG Nechama Gilad
CC Christabel CMY Chan
IP Inbar Plaschkes
WW WS Fred Wong
DE David Engelberg
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ELISA kits for mouse leptin (Proteintech Group), adiponectin (R&D Systems), and albumin (Abcam) were carried out according to manufacturer’s instructions. Lung lysates were prepared as described in “Immunoblotting,” with the only difference being that 1x PBS with Halt Protease and Phosphatase Inhibitor Cocktail was used as lysis buffer.

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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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