In vivo and ex vivo BLI

In vivo BLI was performed according to the method of Edinger et al.¹⁸ Briefly, mice were injected intraperitoneally with luciferin (10 μg/g body weight). Ten minutes later, mice were imaged using an IVIS100 charge-coupled device imaging system (Xenogen) for 5 minutes. Imaging data were analyzed and quantified with Living Image software (Xenogen). Ex vivo BLI was performed according to the method described by Beilhack et al.²³