Cell culture and mice

HTLV-1 immortalized cell lines (MT-2, MT-4), ATL cell lines (MT-1, ATL-2, ATL-T, ATL-43T, ATL-55T, ED, and TL-Om1), T-cell lines not infected with HTLV-1 (Jurkat, Hut78, CEM, and Kit 225), and mouse T-cell line (CTLL-2) were maintained as described previously.²⁷ HepG2, 293T, and COS7 cells were grown in Dulbecco modified Eagle medium supplemented with 10% FBS and antibiotics. Plat-E cells were cultured in Dulbecco modified Eagle medium supplemented with 10% FBS containing 10 μg/mL blasticidin and 1 μg/mL puromycin. EL4 cells were cultured with RPMI 1640 containing 10% FCS, antibiotics, and 50μM 2-mercaptoethanol. C57BL/6J mice were purchased from CLEA Japan. Peripheral blood mononuclear cells were isolated from healthy volunteers under an institutional review board-approved protocol. All animals used in this study were maintained and handled according to protocols approved by Kyoto University.