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Cells were seeded in triplicates in 10% DMEM without antibiotics in 24-well plates 24 h before transfection and counted 24–96 h after transfection. Cell viability was measured using the MTT assay (Sigma Aldrich, Saint Louis, MO, USA). The absorbance was measured on Multi-Detection Microplate Reader (BIO-TEK, Winooski, VT, USA).
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