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After 20‐min. Ang‐II treatment, cells were incubated with 5 μM Fluo3‐AM (Sigma‐Aldrich) at 37°C for 30 min.; then, the cells were washed several times with PBS and further incubated for 40 min. Fluorescent was detected using an inverted microscope.
Copyright and License information: ©2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
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