Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Production of bsAbs

MB Maximilian Bönisch
CS Carolin Sellmann
DM Daniel Maresch
CH Claudia Halbig
SB Stefan Becker
LT Lars Toleikis
BH Björn Hock
FR Florian Rüker
ask Ask a question
Favorite

Each of the four chains was present on a separate pTT5 plasmid. The molar ratio of plasmids during transfection was 2:1:1:1 (B10v5 heavy chain: B10v5 light chain: hu225M or huOKT3 heavy chain: hu225M or huOKT3 light chain). Production of bsAbs in HEK293-6E was performed as described above. Production of bsAbs in Expi293F™ was done using ExpiFectamine™ 293 Transfection kit (gibco, A14524) according to the manufacturer's instructions. Production of bsAbs in ExpiCHO-S was done using the ExpiCHO™ Expression System (gibco, A29133) following the instructions for the high titre protocol. Protein A purification, dialysis and SEC was done as described above.

Copyright and License information:  ©2017 The Author 2017. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A