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The full‐length FOXP4 3′‐untranslated region (3′‐UTR) was cloned into a psi‐CHECK2 vector (Promega) and designated as FOXP4‐3′‐UTR‐WT. A QuikChange Site‐Directed Mutagenesis Kit (Stratagene, San Diego, CA, USA) was used to construct a mutant 3′‐UTR of FOXP4. The predicted target sites (UCCCCACC) were mutated to AGGGCCU. All plasmids were confirmed by DNA sequencing.
Copyright and License information: ©2016 John Wiley & Sons Ltd
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