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Golgi staining and sholl analysis

XC Xin Chen
XJ Xue-Mei Jiang
LZ Lin-Jing Zhao
LS Lin-Lin Sun
MY Mei-Ling Yan
YT You Tian
SZ Shuai Zhang
MD Ming-Jing Duan
HZ Hong-Mei Zhao
WL Wen-Rui Li
YH Yang-Yang Hao
LW Li-Bo Wang
QX Qiao-Jie Xiong
JA Jing Ai
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At the 8th week after 2VO surgery or brain injection, the rats were anesthetized and killed by cervical dislocation. The brains were removed and immediately processed using the FD Rapid GolgiStain Kit (FD Neurotechnologies, Columbia, SC, USA) according to the manufacturer’s protocol. Finally, brain slices were observed by brightfield microscopy using a × 20 objective on a Zeiss Axio Scope A1 microscope. An average of 15–30 neurons were randomly examined in each group from 3 brain slices by an investigator blind to the treatment of the rat. The selected neurons were traced and reconstructed using Image-Pro Plus software, and the dendritic complexity was determined by Sholl analysis.

Copyright and License information:  ©2017 The Author(s)
Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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