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Tissue fixation

VW Viviane Wilms
CK Christine Köppl
CS Chris Söffgen
AH Anna-Maria Hartmann
HN Hans Gerd Nothwang
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Animals were injected with a lethal dose of sodium pentobarbital (Narkodorm, CP-Pharma GmbH, Burgdorf, Germany) and perfused transcardially with phosphate-buffered saline (PBS) containing (mM): 130 NaCl, 7 Na2HPO4, 3 NaH2PO4, pH 7.4, followed by fixation by 4% paraformaldehyde (PFA) in PBS. Animals were then decapitated and the head stored in PFA at 4°C overnight. For dissection of the cochlea, the middle ear cavity was opened and bony structures were removed. For brain and kidney tissue, samples were collected after perfusion of the animal and stored in 30% sucrose and 4% PFA at 4°C overnight.

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