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MTT (tetrazolium dye) proliferation assay
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Panc02 cells (N = 7500) were plated into each well of 96-well plates. Two days later, MTT (Trevigen, Gaithersburg, MD, USA) was added to the cell culture media and incubated for 2 h. The cell culture media were then removed and DMSO was added to dissolve formazan (purple precipitate). The absorbance of each well was read at 540 nm on a plate reader.
Copyright and License information: Springer-Verlag GmbH Germany, part of Springer Nature ©2019
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