Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
The short hairpin RNAs (shRNAs) targeting LINC00511 or PYCR1 (sh‐LINC00511 or sh‐PYCR1) and its negative control shRNA (sh‐NC) were constructed by Genepharm (Shanghai, China). MiR‐4739 inhibitor, mimic, inhibitor‐NC, and mimic‐NC, were synthesized by Switchgear Genomics (USA). Mimics (100 pmol), inhibitor (50 nM) or shRNA (50 nM) were transfected into HCC827 and A549 cells using Lipofectamine2000 (Thermo Fisher Scientific, USA) and collected after 48 h.
Copyright and License information: ©2022 The Authors. published by Wiley Periodicals LLC.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
