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TNF-α and IL-6 Determination
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Tumor necrosis factor-α and IL-6 concentration in the supernatant of cultured primary astrocytes and mixed glial cells stimulated for 24 h with IS (15–60 μM) were performed by an ELISA assay. For this we used commercially available kits for murine TNF-α and IL-6 (e-Biosciences, San Jose, CA, United States) as previously reported (Marzocco et al., 2015).
Copyright and License information: ©2017 Adesso, Magnus, Cuzzocrea, Campolo, Rissiek, Paciello, Autore, Pinto and Marzocco.
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