The distance travelled by the crystal through the X-ray beam can be calculated based on the jet speed (see Table 1) and beam size. Using the upper limit on the measured size of the lysozyme crystals (i.e., 8 × 8 × 8 µm3) and the lowest possible jet speed (accounting for a 5% uncertainty—see Table 1) gives the minimum distance a single crystal could travel whilst still interacting with two consecutive X-ray pulses, spaced 886 ns apart. Assuming that at least 1 µm of the crystal needs to interact with the X-ray beam to generate a diffraction pattern, the minimum distance travelled by a single crystal hit twice by the XFEL beam was calculated. For the fastest jets, multi-hits were only possible in the presence of the maximum FW of the beam.
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